Connect with us

Technology

Kanazawa University research: Chromatin Accessibility: A new avenue for gene editing

Published

on

KANAZAWA, Japan, Feb. 16, 2024 /PRNewswire/ — In a study recently published in Nature Genetics, researchers from Nano Life Science Institute (WPI-NanoLSI), Kanazawa University explore chromatin accessibility, i.e., endogenous access pathways to the genomic DNA, and its use as a tool for gene editing.

Our DNA is protected from unwanted external modifications by forming structures called nucleosomes that consist of threads of DNA wound around chunks of special proteins known as histones. This unique coiled shape prevents the access of undesirable molecules to a cell’s DNA. However, for vital genetic functions—such as DNA repair—the right set of proteins require access to these DNA fragments. This phenomenon known as ‘chromatin accessibility’ involves a privileged set of protein molecules, many of which are still unknown.

Now, researchers from Nano Life Science Institute (WPI-NanoLSI), Kanazawa University, led by Yusuke Miyanari, have used advanced genetic screening methods to unravel chromatin accessibility and its pathways.

For the investigation the team used a combination of two technologies—CRISPR screening and ATAC-see. While the former is a method to suppress the function of a desired set of genes, the latter is a means to identify which ones are essential for chromatin accessibility. Thus, using this method all genes playing a crucial role in chromatin accessibility could be pinned down.

With the help of these assays, novel pathways and individual players involved in chromatin accessibility were uncovered—some playing a positive role and some negative. Of these, one particular protein, TFDP1, showed a negative effect on chromatin accessibility. When it was suppressed, a significant increase in chromatin accessibility was observed, accompanied by nucleosome reduction. A deeper dive into the mechanism of TFDP1 revealed that it functions by regulating the genes responsible for production of certain histone proteins.

The team then focused their study towards exploring biotechnological applications of their findings. After suppressing TFDP1, two different approaches were tried. The first approach involved gene editing using the CRISPR/Cas9 tool. This revealed that deletion of TFDP1 made the gene editing process easier. Now, most chromatin accessibility occurs in nucleosome-depleted regions or NDRs. However, by suppressing TFDP1 chromatin accessibility occurred not only in NDRs but across other regions as well. Secondly, the depletion of TFDP1 aided the process of converting regular cells into stem cells, a massive step forward in cellular transformation.

This study identified new chromatin accessibility pathways and channels for exploring its potential even further. “Our study shows the significant potential to manipulate chromatin accessibility as a novel strategy to enhance DNA-templated biological applications, including genome editing and cellular reprogramming,” conclude the researchers.

Background

CRISPR screening: CRISPR screening is a large-scale tool used to find genes involved in a particular function. In this method, genes from the entire genome can be deleted one at a time to investigate which ones play a role in any target pathway. The mechanisms and roles of the genes narrowed down are then understood further using biological assays. In this study the genes identified by CRISPR screening were subjected to ATAC-see to confirm their involvement with chromatin accessibility.

ATAC-see: Transposase-Accessible Chromatin with visualization (ATAC-see) is a technique whereby accessible DNA locations can be visualized and quantified. ATAC-see involves using an enzyme to insert fluorescent proteins into the DNA at accessible sites. This technique works within nucleosome-depleted regions but also beyond these regions. Hence, when such sites were visualized after running CRISPR screening, the researchers could understand which genes were affecting chromatin accessibility directly.

Reference

Satoko Ishii, Taishi Kakizuka, Sung-Joon Park, Ayako Tagawa, Chiaki Sanbo, Hideyuki Tanabe, Yasuyuki Ohkawa, Mahito Nakanishi, Kenta Nakai, Yusuke Miyanari. Genome-wide ATAC-see screening identifies TFDP1 as a modulator of global chromatin accessibility. Nature Genetics, 2024. Published Feb 15, 2024

DOI :10.1038/s41588-024-01658-1

https://www.nature.com/articles/s41588-024-01658-1

Related figures

https://nanolsi.kanazawa-u.ac.jp/wp/wp-content/uploads/Fig.-1-1.png

Figure 1. a. Screening results of the players involved in chromatin accessibility analyzed by ATAC-see.

b. Successful reprogramming to stem cells (indicated by presence of TRA-1-60) after depletion of TFDP1 (right).

© 2024 Ishii, et al., Nature Genetics

Contact
Hiroe Yoneda
Senior Specialist in Project Planning and Outreach
NanoLSI Administration OfficeNano Life Science Institute (WPI-NanoLSI)
Kanazawa University
Kakuma-machi, Kanazawa 920-1192, Japan
Email: nanolsi-office@adm.kanazawa-u.ac.jp
Tel: +81 (76) 234-4555

About Nano Life Science Institute (WPI-NanoLSI), Kanazawa University

Understanding nanoscale mechanisms of life phenomena by exploring “uncharted nano-realms.”

Cells are the basic units of almost all life forms. We are developing nanoprobe technologies that allow direct imaging, analysis, and manipulation of the behavior and dynamics of important macromolecules in living organisms, such as proteins and nucleic acids, at the surface and interior of cells. We aim at acquiring a fundamental understanding of the various life phenomena at the nanoscale.

https://nanolsi.kanazawa-u.ac.jp/en/

About the World Premier International Research Center Initiative (WPI)

The WPI program was launched in 2007 by Japan’s Ministry of Education, Culture, Sports, Science and Technology (MEXT) to foster globally visible research centers boasting the highest standards and outstanding research environments. Numbering more than a dozen and operating at institutions throughout the country, these centers are given a high degree of autonomy, allowing them to engage in innovative modes of management and research. The program is administered by the Japan Society for the Promotion of Science (JSPS).

See the latest research news from the centers at the WPI News Portal: https://www.eurekalert.org/newsportal/WPI

Main WPI program site:  www.jsps.go.jp/english/e-toplevel

About Kanazawa University

As the leading comprehensive university on the Sea of Japan coast, Kanazawa University has contributed greatly to higher education and academic research in Japan since it was founded in 1949. The University has three colleges and 17 schools offering courses in subjects that include medicine, computer engineering, and humanities.

The University is located on the coast of the Sea of Japan in Kanazawa – a city rich in history and culture. The city of Kanazawa has a highly respected intellectual profile since the time of the fiefdom (1598-1867). Kanazawa University is divided into two main campuses: Kakuma and Takaramachi for its approximately 10,200 students including 600 from overseas.

http://www.kanazawa-u.ac.jp/e/

View original content:https://www.prnewswire.co.uk/news-releases/kanazawa-university-research-chromatin-accessibility-a-new-avenue-for-gene-editing-302063811.html

Continue Reading
Click to comment

Leave a Reply

Your email address will not be published. Required fields are marked *

Technology

Danish Publisher Automates Digital Textbook Delivery with Integrated WooCommerce-Webdoxx Solution

Published

on

By

Danish educational publisher eliminates manual processing errors and delivers instant access to more than 20 digital learning products

LONDON, May 3, 2026 /PRNewswire-PRWeb/ — Forlaget 94, a Danish educational publisher serving commercial colleges and vocational schools since 1994, has transformed its digital textbook distribution by implementing a fully automated WooCommerce-Webdoxx solution.

“Using the Webdoxx-WooCommerce integration we have achieved full automation of order processing, fewer errors, and happier customers,” Tom Gertsen, IT Manager at Forlaget 94

Previously, Forlaget 94 relied on manual processes to distribute digital textbooks to customers. As demand for online educational materials grew, the publisher required a faster, more reliable way to manage orders, provision access, and reduce the risk of administrative errors.

Through its integration of WooCommerce with Webdoxx, Forlaget 94 now runs more than 20 educational products through a 100% automated workflow. The solution automatically processes customer orders and provides instant access to purchased digital textbooks, improving the experience for both customers and internal teams.

“The result is full automation of order processing, fewer errors, and happier customers,” said Tom Gertsen, IT Manager at Forlaget 94 and architect behind the WooCommerce-Webdoxx integration. The automated system has enabled Forlaget 94 to eliminate manual errors, accelerate customer processing, and increase customer satisfaction through immediate access provisioning. The implementation demonstrates how educational publishers can modernize digital content delivery while maintaining secure, managed access to learning materials.

Webdoxx, a service created and managed by Drumlin Security Ltd, provides online DRM and managed document delivery services for publishers, educational organizations, institutions, and commercial content providers.

About Forlaget 94

Forlaget 94 is a Danish educational publisher established in 1994, providing educational products for commercial colleges and vocational schools.

About Webdoxx

Webdoxx is an online DRM and managed document delivery service created and managed by Drumlin Security Ltd. The platform supports secure access to digital publications and documents across a range of sectors, including education, healthcare, government, finance, and publishing.

Media Contact

Mike de Smith, Drumlin Security Ltd, 44 7768404712, info@drumlinsecurity.com, https://www.drumlinsecurity.com/

View original content to download multimedia:https://www.prweb.com/releases/danish-publisher-automates-digital-textbook-delivery-with-integrated-woocommerce-webdoxx-solution-302759942.html

SOURCE Forlaget 94

Continue Reading

Technology

139th Canton Fair Phase 3 Advances Toward a Better Life with New and Strengthened Product Zones

Published

on

By

GUANGZHOU, China, May 3, 2026 /PRNewswire/ — The 139th China Import and Export Fair (Canton Fair) has rolled out nine newly established product zones. Phase 3 features an expanded and upgraded Intelligent Healthcare zone and the inaugural presentation of a Functional & Technical Fabrics zone.

The upgraded Intelligent Healthcare zone brings together 50 companies presenting a full spectrum of intelligent medical solutions, spanning AI-powered diagnostics, surgical robotics, and next‑generation eldercare technologies. Exhibits highlight how medical devices are becoming smaller, more precise, and increasingly non‑invasive. Capsule endoscopy systems demonstrate how gastrointestinal screening can be completed without discomfort, while AI‑enabled traditional Chinese medicine analyzers compress the inspection and inquiry process into minutes. Wearable glucose monitors make chronic disease management easier and more convenient.

Robotic technologies play a prominent role as well. Endoscopic and orthopedic surgical robots showcase enhanced precision through integrated human‑machine coordination, while bionic prosthetic hands use non‑invasive myoelectric sensing to independently control each finger. Intelligent rehabilitation systems, including lower‑limb exoskeletons and hand‑training devices, provide consistent support for patients recovering mobility. Companion‑style eldercare robots, equipped with monitoring and telemedicine functions, signal the rise of integrated home‑based health services.

The debuting Functional & Technical Fabrics zone highlights how the traditional textile industry is moving toward higher-end and smarter products. Exhibitors present materials that combine multi‑layered performance with intelligent responsiveness. Textiles featuring temperature‑regulating fibers, phase‑change materials, and light‑ or heat‑sensitive color‑shifting effects illustrate how fabrics are evolving into adaptive platforms capable of responding to environmental conditions.

Sustainability emerges as a defining theme. Bio‑based fibers, degradable films, recycled polyester, and organic cotton reflect a shift from isolated eco‑products toward full‑chain green manufacturing. High‑performance outdoor and protective applications further shape the narrative. Materials engineered for waterproof breathability, UV resistance, flame retardancy, and long‑term durability address rising demand across sportswear, professional protection, and medical environments. Smart textiles with embedded health‑monitoring modules demonstrate how apparel is beginning to function as a continuous wellness interface.

Both technology‑driven healthcare and advanced textiles are converging around a shared pursuit of a better life. As these advancements continue to evolve, they reflect a manufacturing landscape increasingly shaped by innovation, resilience, and a commitment to improving everyday living.

For pre-registration, please click: https://buyer.cantonfair.org.cn/register/buyer/email?source_type=16

 

View original content to download multimedia:https://www.prnewswire.com/news-releases/139th-canton-fair-phase-3-advances-toward-a-better-life-with-new-and-strengthened-product-zones-302760704.html

SOURCE Canton Fair

Continue Reading

Technology

CupidFeel Insights Show How Shared Interests Affect Initial Connection Outcomes

Published

on

By

New behavioral insights from CupidFeel offer a carefully considered look at how shared interests influence whether an initial connection on a dating platform is sustained or abandoned in those first critical exchanges.

GIBRALTAR, May 3, 2026 /PRNewswire-PRWeb/ — The findings by CupidFeel are not dramatic, but they are telling. People who referenced a shared interest — whether a genre of music, a type of cuisine, a sport, a creative practice, or even a shared discomfort with small talk — within the first few exchanges of a new conversation were found to be measurably more likely to continue that conversation beyond the initial contact window. The effect was not uniform across all interest categories; certain types of shared interest appeared to carry more relational weight than others.

It was also observed by CupidFeel that the timing of when shared interests entered a conversation mattered. Connections where common ground was discovered organically — through the natural flow of exchange rather than prompted by a profile field or a direct question — showed stronger indicators of sustained interest. The discovery, in other words, carried more meaning when it felt like something found rather than something declared.

Among the most quietly striking findings in the CupidFeel data was the role of specificity. Broad shared categories — “we both like travel,” “we both enjoy cooking” — were associated with polite, often brief exchanges that rarely extended past pleasantries. But when specificity entered the picture — when one person mentioned a particular documentary that had stayed with them, or a city they had visited and could not stop thinking about — the conversational energy shifted. Something opened up.

In a CupidFeel review of trends in profile engagements, those whose profiles reflected specific, idiosyncratic interests — rather than broadly appealing ones — also showed higher rates of receiving first messages, a finding that runs gently counter to the instinct many people have to present themselves in the most universally appealing terms possible.

What seemed to matter most was not the quantity of overlap but whether the overlap that existed was felt — whether it produced a sense of being seen in some particular, non-generic way. A CupidFeel review of early conversation patterns suggests that a single deeply resonant shared interest may be more generative for early connection than a long list of surface-level commonalities that, taken together, feel more like a demographic profile than a person.

About CupidFeel

CupidFeel is an online dating platform built around the belief that meaningful connections begin with emotional honesty and the willingness to let a conversation go somewhere real. It came into being for people who are less interested in the mechanics of dating and more drawn to the possibility of something that feels grounded — exchanges that move at their own pace, guided by genuine curiosity rather than performance.

A CupidFeel review of its own design principles returns consistently to the same question: what does it take for a first message to feel like it might be worth the journey? The platform makes room for the kind of interaction that doesn’t always have a clear destination but feels, from the first exchange, like something real. CupidFeel is a place where the unexpected is not something to be managed, but something to be welcomed.

Media Contact

Timothy Albers, CupidFeel, 1 14845691657, smm@cupidfeel.com, https://cupidfeel.com/

View original content:https://www.prweb.com/releases/cupidfeel-insights-show-how-shared-interests-affect-initial-connection-outcomes-302759951.html

SOURCE CupidFeel

Continue Reading

Trending