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Kanazawa University research: High speed atomic force microscopy studies provide insights into influenza A viral replication

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KANAZAWA, Japan, July 29, 2024 /PRNewswire/ — Researchers at Nano Life Science Institute (WPI-NanoLSI), Kanazawa University, IMDEA Nanoscience (Madrid, Spain) and CNB-CSIC (Madrid, Spain) report in ACS Nano experiments that reveal a cycle of conformational stages that recombinant Influenza A genomes pass through during RNA synthesis.

Influenza A is a global health risk responsible for local epidemics and deadly pandemics. As such, the mechanism by which the virus replicates itself has attracted significant interest. Researchers led by Shingo Fukuda at Nano Life Science Institute (WPI-NanoLSI) at Kanazawa University in Japan, Jaime Martin-Benito at CNB-CSIC and Borja Ibarra  at IMDEA Nanociencia in Spain, used high-speed atomic force microscopy and electron microscopy to pin down the conformational dynamics of recombinant viral genomes (or rRNPs) during RNA synthesis.

Previous attempts to understand what possible conformational changes occur during Influenza A viral multiplication cycle had been hindered by what the researchers describe as the “bulky double-helical structure” of the viral RNPs (vRNP, Fig.1 A), which made it hard to see what was going on. As a result the researchers produced a circular recombinant ribonucleoprotein complex (rRNP) (Fig.1 B), which allowed them to overcome the ‘bulky issue’. The authors used HS-AFM to follow conformational changes of individual rRNP complexes in real-time during active RNA synthesis (Fig.1 C).

Their work provides first direct experimental evidence showing that individual rRNPs can be recycled for multiple transcription and replication cycles. This is a key feature for viral multiplication. In addition, their study highlights how factors that affect the stability of the secondary structures of the nascent RNA affect the rate of RNA synthesis.

The authors concluded that the approach is useful for investigating viral transcription and replication mechanisms. “Transcriptional pausing is an intrinsic property of most RNA polymerases, and its regulation constitutes one of the central mechanisms of control of gene expression,” they add. “Future single-molecule experiments of real-time RNA synthesis kinetics by the IAV RdRp [influenza A virus RNA dependent RNA polymerase] within the context of the RNP will help to elucidate the nature of putative pause states and their roles in viral transcription and replication.”

Figure

https://nanolsi.kanazawa-u.ac.jp/wp/wp-content/uploads/Figure-1-2.jpg

Caption : Fig.1 (A, B) HS-AFM images of vRNP (A) and rRNP (B). (C) Successive HS-AFM images of rRNP during RNA synthesis. © 2024 Carlero, et al. Published by American Chemical Society

Glossary

RNA synthesis

Ribonucleic acid (RNA) is a polymer and a nucleic acid. Its replication is catalyzed by an enzyme known as RNA polymerase. The synthesis only proceeds in the presence of the nucleotide and proteins required to build up the RNA molecule.

Although RNA synthesis can use DNA as the synthesis template, a number of viruses replicate with RNA as the template. The enzyme that catalyzes this type of synthesis is known as RNA dependent RNA polymerase.

Recombinant ribonucleic proteins

Recombinant ribonucleoproteins are useful for ways of studying RNA processes. In this instance the authors used a recombinant ribonucleoprotein made up of the same protein components but just 352 nucleotides long, where the RNA segment used had been shown to avoid supercoiling.

High-speed atomic force microscopy

This imaging technique uses a nanosized tip at the end of a cantilever that is scanned over a sample. It can be used to determine the topography of a sample surface from the change in the strength of forces between the tip and the sample with distance, and the resulting deflection of the cantilever. It was first developed in the 1980s but a number of modifications have augmented the functionality of the technique since. It is better suited to imaging biological samples than the scanning tunneling microscope that had been developed because it does not require a conducting sample.

In the 2000s Toshio Ando at Kanazawa University was able to improve the scanning speed to such an extent that moving images could be captured. This allowed people to use the technique to visualize molecular processes for the first time.

Reference

Diego Carlero, Shingo Fukuda, Rebeca Bocanegra, Toshio Ando, Jaime Martin-Benito, and Borja Ibarra Conformational Dynamics of Influenza A Virus Ribonucleoprotein Complexes during RNA Synthesis ACS Nano 2024.

DOI:10.1021/acsnano.4c01362

URL: https://pubs.acs.org/doi/10.1021/acsnano.4c01362

Funding acknowledgements

This work was supported by the NanoLSI Visiting Fellows Program 2019 (to B.I.), the World Premier International Research Center Initiative (WPI), MEXT (Japan), and grants PGC2018-099341-B-I00 (to B.I.), PID2021-126755NB-I00 (to B.I.), and PID2020-117752RB-I00 (to J.M.B.) financed by MCIU/AEI/10.13039/501100011033 and FEDER, UE,  Grant TED2021-132748B-I00 financed by the European Union “NextGeneration EU”/PRTR (to J.M.B.), and Grant No. 20K15140 financed by JSPS KAKENHI (to S.F.). IMDEA Nanociencia acknowledges support from the Severo Ochoa Program for Centers of Excellence in R&D (CEX2020-001039-S).

Contact

Hiroe Yoneda (Ms)
Senior Specialist in Project Planning and Outreach
NanoLSI Administration Office, Nano Life Science Institute (WPI-NanoLSI)
Kanazawa University
Kakuma-machi, Kanazawa 920-1192, Japan
Email: nanolsi-office@adm.kanazawa-u.ac.jp
Tel: +81 (76) 234-4555

About Nano Life Science Institute (WPI-NanoLSI), Kanazawa University

Understanding nanoscale mechanisms of life phenomena by exploring “uncharted nano-realms”

Cells are the basic units of almost all life forms. We are developing nanoprobe technologies that allow direct imaging, analysis, and manipulation of the behavior and dynamics of important macromolecules in living organisms, such as proteins and nucleic acids, at the surface and interior of cells. We aim at acquiring a fundamental understanding of the various life phenomena at the nanoscale.

https://nanolsi.kanazawa-u.ac.jp/en/

About the World Premier International Research Center Initiative (WPI)

The WPI program was launched in 2007 by Japan’s Ministry of Education, Culture, Sports, Science and Technology (MEXT) to foster globally visible research centers boasting the highest standards and outstanding research environments. Numbering more than a dozen and operating at institutions throughout the country, these centers are given a high degree of autonomy, allowing them to engage in innovative modes of management and research. The program is administered by the Japan Society for the Promotion of Science (JSPS).

See the latest research news from the centers at the WPI News Portal: https://www.eurekalert.org/newsportal/WPI

Main WPI program site: 

www.jsps.go.jp/english/e-toplevel

About Kanazawa University

As the leading comprehensive university on the Sea of Japan coast, Kanazawa University has contributed greatly to higher education and academic research in Japan since it was founded in 1949. The University has three colleges and 17 schools offering courses in subjects that include medicine, computer engineering, and humanities.

The University is located on the coast of the Sea of Japan in Kanazawa – a city rich in history and culture. The city of Kanazawa has a highly respected intellectual profile since the time of the fiefdom (1598-1867). Kanazawa University is divided into two main campuses: Kakuma and Takaramachi for its approximately 10,200 students including 600 from overseas.

http://www.kanazawa-u.ac.jp/en/

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How Cool Is Shenzhen? Just Ask Global Journalists Who Come to See It

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SHENZHEN, China, Sept. 4, 2026 /PRNewswire/ — “Shenzhen may well be the world’s No. 1 innovation hub. And together, Shenzhen and Hong Kong form the perfect endpoint of the modern Silk Road.”

That was the glowing verdict American economist Jeffrey Sachs delivered in Shenzhen just over a month ago, effectively giving the city one of the highest-profile stamps of approval in the world of development economics.

Sachs is hardly an ordinary commentator. One of the world’s most influential authorities on sustainable development, and for years a special adviser to the UN secretary-general, he knows a thing or two about how cities and economies grow.

In a recent exclusive interview with Zhi News (from Shenzhen Media Group), Sachs shared his in-depth views. The following is the full report:

when representatives from APEC economies come to Shenzhen, they are bound to be impressed. They will discover that the city is not merely a great place to find capital and business partners, but also a window into entirely new models of development—and perhaps a glimpse of what the future could look like.

After decades of trailblazing development, Shenzhen has more than earned that kind of academic attention. It is, after all, a city built on miracles and a straight-A student in the school of urban development.

The more you admire Shenzhen, the more you begin to understand why it deserves admiration. That, to me, is precisely where the real significance of Shenzhen’s development lies: not simply in what the city has achieved, but in what its experience tells us about where the world may be heading.

As a journalist, I would put it this way: Shenzhen is a mother lode for anyone trying to understand China. For global academia, and perhaps even more so for the world’s media, it is a gold mine of stories, ideas and opportunities.

Over the past year or two, a curious phenomenon has swept across Western social media and, increasingly, Western society itself: China-maxxing, sometimes jokingly described as “becoming Chinese.”

“You met me at a very Chinese time in my life.”

Yes, that viral internet phrase borrows its punchline from the Hollywood classic Fight Club. Earlier this year, The New York Times offered a wonderfully tongue-in-cheek description of the trend in one of its features:  If you drink hot water, wear slippers indoors or shop at Asian supermarkets, you may be Chinese, according to the internet.

But here is what caught my attention.

When it comes to Shenzhen—and China more broadly—Western mainstream media can sometimes be surprisingly slow on the uptake. Take China-maxxing. It only became a mainstream media story, splashed across newspapers and television screens, earlier this year—more than six months after a Twitter user, @Girl_virus, first posted about “becoming Chinese.”

To put it bluntly, China-maxxing was not exactly handed down to the mainstream media from above. The world’s netizens pushed it up there.

The New York Times even framed the provocative question this way in a headline: From Labubu to “becoming Chinese,” has China become cool?

Well, if the Times’ reporter comes to Shenzhen, I’ll make a pretty safe bet: he or she will come away thinking, Shenzhen is super cool.

So here’s my advice to my fellow journalists around the world: follow the lead of those pioneering scholars. Get closer to Shenzhen. Walk into Shenzhen. Stay a little longer. Look a little deeper. And then go farther afield.

There are simply too many fascinating stories here waiting to be told—and too many forces behind those stories waiting to be decoded.

And here’s the best part.

More than two decades ago, Shenzhen residents already had the confidence to put it into one unforgettable slogan:

“Once you come, you’re a Shenzhener.”

I suspect foreign correspondents arriving in Shenzhen today may discover their own version of that old Shenzhen experience:

Come for the story. Stay for the experience. Leave having become, at least a little bit, a Shenzhener.

And trust me, it’s going to be quite an experience. 

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SOURCE Shenzhen Media Group

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Datamaran Appoints Bert Sinnema as Chief Technology Officer

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LONDON, NEW YORK and LEEUWARDEN, Netherlands, Sept. 4, 2026 /PRNewswire/ — Datamaran, the AI-powered risk and governance software provider, today announced the appointment of Bert Sinnema as Chief Technology Officer. Based out of the company’s Leeuwarden office in the Netherlands, Sinnema will lead Datamaran’s global engineering and product teams, including the engineering hub in Valencia, Spain, driving the technology and product vision behind the platform that helps organizations understand and manage evolving risks and opportunities.

As CTO, Sinnema will own Datamaran’s product vision, strategy, and technology roadmap, from setting direction to architecture, delivery, and the teams building it. He steps into the role at a pivotal moment for the company, as Datamaran deepens its use of AI across the platform to give customers faster, more decision-ready insights into risk, regulation, and stakeholder expectations. His mandate: lead the engineering and product organization, accelerate the pace of AI-driven product development, and ensure the platform keeps pace with the increasingly complex and fast-moving risk landscape.

A track record scaling product engineering teams

Sinnema brings more than 12 years of product engineering leadership experience spanning startups, scale-ups, and category-leading platforms, with deep expertise at the intersection of engineering, cybersecurity, and AI. He joins Datamaran from Eye Security, Europe’s fastest-growing cybersecurity company, where he served as VP of Engineering and built the product and engineering organization from the ground up, scaling the multi-hub international team 3x and helping the company reach a €60M Series C round.

Before Eye Security, Sinnema spent over three years at HackerOne, the world’s leading ethical hacking and bug bounty platform, rising to Director of Engineering. Earlier in his career, Sinnema founded and served as CTO of SmartLockr, a secure communications and privacy platform he bootstrapped, scaled across three countries, and took through Microsoft’s Ventures Accelerator in Berlin.

Building for Datamaran’s next chapter

“Bert has spent his career building engineering organizations from the ground up and scaling them through some of the most demanding environments, including as a founder himself. He knows firsthand what it takes to turn conviction into something real, and to keep adapting as the market evolves,” said Marjella Lecourt-Alma, CEO and co-founder of Datamaran. “As Datamaran evolves from ESG issue management into an AI-powered governance platform, Bert’s founder mindset and experience building teams, technology, and culture make him exactly the right leader to shape what comes next for our teams, customers, and partners.”

“I like to operate at the intersection of product engineering leadership and AI, where the hardest problems live, and the highest-leverage decisions get made,” said Sinnema. “Datamaran is applying AI to help organizations navigate a genuinely complex risk landscape, and I’m excited to bring my experience building and scaling engineering and product organizations to that mission. This is the most interesting time to be building in tech, and I’m looking forward to being in the middle of it with the team.”

Contact: Helen Skeen, Director of Communications and Insights: helen.skeen@datamaran.com.

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Diving deeper into real-life home demands, EZVIZ brings next-generation home innovations and products to IFA with expanded application scenarios

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HOOFDDORP, Netherlands, Sept. 4, 2026 /PRNewswire/ — This IFA, EZVIZ explores new frontiers with its full-range products in the smart home hall 1.2. Under the theme “Accelerating Innovation”, the company creates an immersive experience at booth 175, featuring anticipated debuts that highlight its leadership in self-developed smart home security and home entry products, and introducing next-generation devices powered by local-first EZVIZ AI. EZVIZ also builds excitement by unveiling brand-new smart cleaning robots and wearable electronics for stay-at-home and on-the-go lifestyle needs.

“EZVIZ has prepared a technological feast, with each line-up full of hardcore innovations,” said Chris Xu, General Manager of EZVIZ’s International Business Center. “Especially in Europe, where we’ve developed our portfolio and services for over a decade, we offer global solutions and Europe-tailored options rooted in insights and localization.”

As the hall’s largest stand, EZVIZ designs 5 interlinked areas, allowing audiences to embark on a journey from visual protection and add-on home convenience to robotic fun and future imagination.

For its renowned smart home cameras, EZVIZ aims to lead with an innovative leap in technology and product design.

The triple-lens 90f series for ultra-vision: Upgraded from the 90 Duals, the wired H90f and battery-powered HB90f pack three lenses, ushering in the triple-lens era of professional-level awareness. They are designed to cover visible distance with 12x mixed zoom, capturing details that are easy to miss across broad areas like ranches.The dual-lens 3K series for compact, mobile security: With the basic CB30 and advanced CB60 models, this series embodies flexibility enabled by high-capacity batteries and magnetic bases. The CB60 further maintains a clear view beyond Wi-Fi range with 8x zoom and switchable 4G-WiFi connectivity.

EZVIZ further expands the potential of smart cameras with EZVIZ AI, delivering the EZVIZ MIRA advanced model system directly on devices to unlock advanced detection and management.

EZVIZ AI CoreX home station: Bringing EZVIZ MIRA to the local level, EZVIZ delivers uncompromised AI performance through the masterpiece edge model AI CoreX. Acting as a private home station without reliance on the cloud, it offers large file storage, smart search with text, photos and videos, and multi-device management, without needing to be online.Local-first AI solutions: Making local AI features accessible at all levels, EZVIZ integrates the power of local AI chips on end devices with AI capabilities on edge devices, and by offering flexible AI home plans. Users can either enable detection features on standalone cameras without subscriptions, or customize scalable systems by adding AI CoreX or other home hubs like the X6.

In smart entry, EZVIZ introduces new line-ups for broader scenarios.

Smart AI locks: From DIY options to high-end choices, EZVIZ offers a complete understanding of smart locks. Among them is the flagship DL50FVX, integrated with pioneering technologies. It provides on-device palm vein and facial unlocking, enhanced awareness with dual lenses and a built-in screen.Doorbells & peephole cameras with add-on AI: EZVIZ showcases versatile options, from door viewers to dual-lens video doorbells that keep entrances connected. The state-of-the-art EP8 Ultra marks a new chapter in video doorbells, providing thoughtful care beyond security with its interactive local AI screen.Smart entry systems for villas: Designed for interaction at gates and driveways, EZVIZ presents systematic solutions with the representative HP7 Pro Video Doorphone and HG2 600 Pro Gate Opener. The HP7 Pro ditches bulky intercoms, offering keyless entry, 4K awareness and centralised management, while the HG2 600 Pro delivers lag-free vehicle access.

For more information, please visit ezviz.com/page/ifa2026.

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